| Description |
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation (1-4). MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones, and neurotransmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK) (figure 1) (5,6). Both kinases are known to weakly autophosphorylate on tyrosine (5). The p44/42 MAP Kinase Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of p44 and p42 MAP Kinases (Erk1 and Erk2). The kit includes a phospho-MAP kinase antibody that recognizes threonine 202 and tyrosine 204 phosphorylated MAP kinase, a control MAP kinase antibody (phosphorylation-state independent), protein controls for western blots and the HRP
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