HeLa cell extracts were spiked with 5μl of Rac1ΔC protein preloaded with GDP (D) or GTP-γ-S (T) as described above, and subjected to a Rac-GTP pulldown.
Mouse neutrophil granulocytes were stimulated with the N-formylated peptide fMLP for the indicated time periods and subjected to a Rac-GTP pulldown. Note that the antibody provided in the kit detects specifically Rac1. See protocol for details.