Example positive control sample standard curve. A dilution series of extract in Incubation Buffer in the working range of the assay. The extract was prepared from Hek293T cells treated with Etoposide by direct lysis.
Example experimental analysis of drug treatment of Hek 293T and MCF7 cells. Cells were treated with Camptothecin, Etoposide or drug’s vehicle, as indicated. Diluted cell extracts were analyzed by this kit (A, C) and p53 protein ELISA (B, D), using ab117995). Extract of Hek 293T treated with Etoposide was used for positive control sample standard curves. Relative levels interpolated from standard curves are shown.
The p53 (pSer9) ELISA specifically measures the phosphorylated Serine. Extracts of Hek 293T cells (induced with Etoposide) were treated with increasing concentrations of λ protein phosphatase or left untreated (Contr), and phospho Ser9 (in red) and total p53 protein (in green) levels were determined, respectively, using this kit and ab117995. Dilutions of extracts of Hek 293T cells treated with Etoposide were used to construct the standard curves.
All lanes : p53 (pSer9) Human ELISA Kit (ab131384)Lane 1 : Mol Wt MarkersLane 2 : Extracts of MCF7 cells were treated with vehicle at 20 µgLane 3 : Extracts of MCF7 cells were treated with Camptothecin at 20 µgLane 4 : Extracts of Hek293T cells were treated with vehicle at 20 µgLane 5 : Extracts of Hek293T cells were treated with Etoposide at 20 µgLane 6 : Extracts of Hek 293T cells (induced with Etoposide) were left untreated at 8 µgLane 7 : Extracts of Hek 293T cells (induced with Etoposide) were treated with λ protein phosphatase (400x diluted) at 8 µgLane 8 : Extracts of Hek 293T cells (induced with Etoposide) were treated with λ protein phosphatase (100x diluted) at 8 µgLane 9 : Extracts of Hek 293T cells (induced with Etoposide) were treated with λ protein phosphatase (25x diluted) at 8 µg
All lanes : p53 (pSer9) Human ELISA Kit (ab131384)Lane 1 : Mol Wt MarkersLane 2 : Extracts of MCF7 cells were treated with vehicle at 20 µgLane 3 : Extracts of MCF7 cells were treated with Camptothecin at 20 µgLane 4 : Extracts of Hek293T cells were treated with vehicle at 20 µgLane 5 : Extracts of Hek293T cells were treated with Etoposide at 20 µgLane 6 : Extracts of Hek 293T cells (induced with Etoposide) were left untreated at 8 µgLane 7 : Extracts of Hek 293T cells (induced with Etoposide) were treated with λ protein phosphatase (400x diluted) at 8 µgLane 8 : Extracts of Hek 293T cells (induced with Etoposide) were treated with λ protein phosphatase (100x diluted) at 8 µgLane 9 : Extracts of Hek 293T cells (induced with Etoposide) were treated with λ protein phosphatase (25x diluted) at 8 µg