Example of a typical p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
Example of a typical p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of p38 MAPKα (pT180/Y182) are normalized and plotted.
Cell line analysis for Total p38 MAPKα from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
Induction of p38 MAPKα (pT180/Y182) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of p38 MAPKα (pT180/Y182) are plotted and compared against Total p38 MAPKα protein levels. Comparative p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) data also shown by Western Blot.