NF-kappaB p65 Total PhosphoTracer ELISA Kit

Name	NF-kappaB p65 Total PhosphoTracer ELISA Kit
Supplier	Abcam
Catalog	ab119637
Category	ELISA Kit
Assay Type	Semi-quantitative
Sample Type	Cell culture extracts
Detection	Fluorescent
Sensitivity	10 µg protein/ml
Range	10 µg protein/ml - 1000 µg protein/ml
Format	Microplate
Applications	ELISA
Species Reactivities	Mouse, Human, Rat
Gene	RELA
Supplier Page	Shop

Product images

Detection of total NF-kappaB p65, using ab119637 on HeLa cells stimulated with various concentrations of TNF alpha.

Using the PhosphoTracer Total NF-kappaB p65 assay, the amount of lysate required to detect NF-kappaB p65 in TNFalpha-treated HeLa cell lysates was examined. Up to 50 μg of lysate/well was loaded into replicate wells of a PhosphoTracer microplate, and analysed for total NF-kappaB p65. Total NF-kappaB p65 was readily detected in less than 10 μg of lysate/well.

Using the NF-kappaB p65 assay kits, a significant stimulation of NF-kappaB p65 phosphorylation at Ser536 is detected in HeLa cells treated with TNFalpha for 10 minutes (+) compared with untreated cells (-), while no change in total NF-kappaB p65 levels is observed.

Using Western blot, a significant stimulation of NF-kappaB p65 phosphorylation at Ser536 is detected in HeLa cells treated with TNFalpha for 10 minutes (+) compared with untreated cells (-), while no change in total NF-kappaB p65 levels is observed.