Native Human Lactoferrin protein was measured in serum diluted 1:64 and urine diluted 1:8 in Sample Diluent NS prior to a 2-fold dilution series in Sample Diluent NS. Purified Human Lactoferrin protein was spiked into cell culture media diluted 1:20 prior to a fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Native Human Lactoferrin protein was measured in citrate plasma (1:128), EDTA plasma (1:150), and heparin plasma (1:64) diluted in a 2-fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Ten individual healthy male donors and one pooled sample (n = 50) were evaluated for the presence of Lactoferrin in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (1:75). In the 10 individual donors, the mean level of Lactoferrin is 350.3 ng/mL with a range of 106.2 – 627.7 ng/mL and a standard deviation of 159.4 ng/mL.