
Standard curve of msIL-6 in different diluents with background signal subtracted (duplicates; +/- SD).

IL-6 measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).

msIL-6 measured in biological fluids, background signal subtracted (duplicates +/- SD).

IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

Representative standard curve using ab100712

Representative standard curve using ab100712