
Standard curve of human IL-6 with background signal subtracted (duplicates; +/- SD).

IL-6 measured in cell culture supernatants from control or treated (48 hours with 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD)

IL-6 detected in positive control (Pos CTRL; ab46027), and in supernatants from control cells or cells stimulated for 24 hours with 50 nanogram x mL-1 of PMA (ab120297) with 1 microgram x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS).

Representative Standard Curve using ab46027

Dilution curves of human IL-6 (ab46027) and NIBSC standard (89/548). One ng of standard IL-6 corresponds to 87.2 (+/- 8.0) IU NIBSC standard. Background signal subtracted (duplicates; +/- SD).