Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-BLM Antibody, Affinity Purified:
Cat. No. PLA0029: Detection of Human BLM by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BLM Antibody
PLA0029 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
ImmunoblottingRabbit Anti-BLM Antibody, Affinity Purified:
Cat. No. PLA0029: Detection of Mouse BLM by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-BLM Antibody
PLA0029 used for WB at 2 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
ImmunoprecipitationRabbit Anti-BLM Antibody, Affinity Purified:
Cat. No. PLA0029: Detection of Human BLM by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BLM antibody
Cat. No. PLA0029 used for IP at 6 μg/mg lysate. BLM was also immunoprecipitated by a BLM Antibody B and C. For blotting immunoprecipitated BLM
PLA0029 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.