Rabbit anti-RNA Polymerase II Antibody, Affinity Purified

Name	Rabbit anti-RNA Polymerase II Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0127
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Zebrafish, Rat, Dog, Bovine, Rabbit, Primate, Mouse, Human, Amphibians, Monkey, Pig
Gene	POLR2A
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-RNA Polymerase II Antibody, Affinity Purified: <B>Cat. No. PLA0127</B>: Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg for WB), HeLa (Lane H; 50 μg), Jurkat (Lane J; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-RNA Polymerase II Antibody <B>PLA0127</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0127: Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg for WB), HeLa (Lane H; 50 μg), Jurkat (Lane J; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-RNA Polymerase II Antibody PLA0127 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-RNA Polymerase II Antibody, Affinity Purified: <B>Cat. No. PLA0127</B>: Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from 293T cells. Antibody: Affinity purified Rabbit Anti-RNA Polymerase II antibody <B>Cat. No. PLA0127</B> used for WB at 1 μg/mL and used for IP at 6 μg/mg lysate. RNA Polymerase II was also immunoprecipitated by RNA Polymerase II Antibody B. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation
Rabbit Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0127: Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from 293T cells. Antibody: Affinity purified Rabbit Anti-RNA Polymerase II antibody Cat. No. PLA0127 used for WB at 1 μg/mL and used for IP at 6 μg/mg lysate. RNA Polymerase II was also immunoprecipitated by RNA Polymerase II Antibody B. Detection: Chemiluminescence with an exposure time of 10 seconds.