Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-Chk1 Antibody, Affinity Purified:
Cat. No. PLA0054: Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (30 μg) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody: Affinity purified goat Anti-Chk1 (
Cat. No. PLA0054) used at 0.1 μg/mL. Detection: Chemiluminescence with 30 second exposure.