Rabbit anti-p300 Antibody, Affinity Purified

Name	Rabbit anti-p300 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0096
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Monkey, Chimpanzee, Human, Ape, Primate
Gene	EP300
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-p300 Antibody, Affinity Purified: <B>Cat. No. PLA0096</B>: Detection of Human p300 by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-p300 (<B>Cat. No. PLA0096</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-p300 Antibody, Affinity Purified: Cat. No. PLA0096: Detection of Human p300 by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-p300 (Cat. No. PLA0096) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-p300 Antibody, Affinity Purified: <B>Cat. No. PLA0096</B>: Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-p300 Antibody <B>PLA0096</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoblotting
Rabbit Anti-p300 Antibody, Affinity Purified: Cat. No. PLA0096: Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-p300 Antibody PLA0096 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-p300 Antibody, Affinity Purified: <B>Cat. No. PLA0096</B>: Detection of Human p300 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-p300 antibody <B>Cat. No. PLA0096</B> used for IP at 6 μg/mg lysate. p300 was also immunoprecipitated by p300 Antibody B. For blotting immunoprecipitated p300, <B>PLA0096</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 1 second.

Immunoprecipitation
Rabbit Anti-p300 Antibody, Affinity Purified: Cat. No. PLA0096: Detection of Human p300 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-p300 antibody Cat. No. PLA0096 used for IP at 6 μg/mg lysate. p300 was also immunoprecipitated by p300 Antibody B. For blotting immunoprecipitated p300, PLA0096 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 1 second.