Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-RFC1 Antibody, Affinity Purified:
Cat. No. PLA0047: Detection of Human RFC1 by Western Blot and Immunoprecipitation. Samples: Nuclear extract from HeLa cells (25 μg for WB; 500 μg for IP). Antibodies: Affinity purified goat Anti-RFC1 (
Cat. No. PLA0047) used at 0.5 μg/mL for WB and 3 μg/0.5 mg lysate for IP or a control (Ctrl) Antibody to RFC1 used at 3 μg/0.5 mg lysate for IP. Detection: Chemiluminescence.