Rabbit anti-BCR Antibody, Affinity Purified

Name	Rabbit anti-BCR Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0233
Prices	$323.50
Sizes	100 µl
Applications	FC
Species Reactivities	Ape, Human
Gene	BCR
Supplier Page	Shop

Product images

<B>Immunocytochemistry</B><BR/>Rabbit Anti-BCR Antibody, Affinity Purified: <B>Cat. No. PLA0233</B>: Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified Rabbit Anti-BCR (<B>Cat. No. PLA0233</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: Green-fluorescent goat Anti-Rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.

Immunocytochemistry
Rabbit Anti-BCR Antibody, Affinity Purified: Cat. No. PLA0233: Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified Rabbit Anti-BCR (Cat. No. PLA0233) used at a dilution of 1:1,000 (1 μg/mL). Detection: Green-fluorescent goat Anti-Rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Flow Cytometry</B><BR/>Rabbit Anti-BCR Antibody, Affinity Purified: <B>Cat. No. PLA0233</B>: Flow Cytometric Detection of BCR. 1 × 10<SUP>6</SUP> K562 cells were fixed, permeabilized, and stained with 0.25 μg Anti-BCR <B>PLA0233</B> in a 150 mcl reaction. Isotype Anti-KLH control (black), no Antibody (red) and Anti-BCR <B>PLA0233</B> (blue).

Flow Cytometry
Rabbit Anti-BCR Antibody, Affinity Purified: Cat. No. PLA0233: Flow Cytometric Detection of BCR. 1 × 10⁶ K562 cells were fixed, permeabilized, and stained with 0.25 μg Anti-BCR PLA0233 in a 150 mcl reaction. Isotype Anti-KLH control (black), no Antibody (red) and Anti-BCR PLA0233 (blue).