Rabbit anti-E2F1 Antibody, Affinity Purified

Name	Rabbit anti-E2F1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0139
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Dog, Rabbit, Guinea Pig, Monkey, Primate, Human, Rat, Mouse, Bovine, Pig, Horse
Gene	E2F1
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-E2F1 Antibody, Affinity Purified: <B>Cat. No. PLA0139</B>: Detection of Human E2F1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-E2F1 Antibody (<B>Cat. No. PLA0139</B>) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). E2F1 was also immunoprecipitated using Rabbit Anti-E2F1 Antibody that recognizes a different epitope at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunoblotting
Rabbit Anti-E2F1 Antibody, Affinity Purified: Cat. No. PLA0139: Detection of Human E2F1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-E2F1 Antibody (Cat. No. PLA0139) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). E2F1 was also immunoprecipitated using Rabbit Anti-E2F1 Antibody that recognizes a different epitope at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).