Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Phospho SMC1 (S957) Antibody, Affinity Purified:
Cat. No. PLA0012: Detection of Human Phospho SMC1 (Ser957) by Western Blot. Sample: Whole cell extracts from HeLa cells (30 μg/lane) that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Irradiated lysate was then treated with calf intestinal phosphatase (CIP) or left untreated. Antibody: Affinity purified Rabbit Anti-SMC1 pSer957
PLA0012 used at 0.1 μg/mL. Detection: Chemiluminescence with 30 second exposure.