| Detection |
Store kit reagents between 4°C.Immediately after use remaining reagents should be returned to cold storage (4°C). All reagents should be warmed to room temperature before use. BCIP/NBT buffer is potentially carcinogenic and should be disposed of appropriately, caution should be taken when handling this reagent, always wear glovesCapture Antibody is supplied sterile: once opened keep the vial sterile or aliquot and store at -20oC. For optimal performance prepare the Capture Antibody dilution immediately before use. If not used within a short period of time, reconstituted Detection Antibody should be aliquoted and stored at -20°C. In these conditions the reagent is stable for at least one year. For optimal performance prepare the Streptavidin-AP dilution immediately prior to use. Do not keep this solution for further experiments.
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Figure 1; Principle of assay