Rabbit anti-Phospho SMC1 (S966) Antibody, Affinity Purified

Name	Rabbit anti-Phospho SMC1 (S966) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0015
Prices	$345.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Primate, Ape, Human, Marmoset, Monkey
Gene	SMC1A
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho SMC1 (S966) Antibody, Affinity Purified: <B>Cat. No. PLA0015</B>: Detection of Human Phospho SMC1 (S966) by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-Phospho SMC1 (S966) (<B>Cat. No. PLA0015</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho SMC1 (S966) Antibody, Affinity Purified: Cat. No. PLA0015: Detection of Human Phospho SMC1 (S966) by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-Phospho SMC1 (S966) (Cat. No. PLA0015) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunocytochemistry</B><BR/>Rabbit Anti-Phospho SMC1 (S966) Antibody, Affinity Purified: <B>Cat. No. PLA0015</B>: Detection of Human Phospho SMC1 (S966) by Immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-Phospho SMC1 (S966) (<B>Cat. No. PLA0015</B>)used at a dilution of 1:5,000 (0.2 μg/mL). Detection: Red fluorescent Anti-Rabbit IgG - DyLight<SUP>®</SUP> 594 conjugate used at a dilution of 1:100.

Immunocytochemistry
Rabbit Anti-Phospho SMC1 (S966) Antibody, Affinity Purified: Cat. No. PLA0015: Detection of Human Phospho SMC1 (S966) by Immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-Phospho SMC1 (S966) (Cat. No. PLA0015)used at a dilution of 1:5,000 (0.2 μg/mL). Detection: Red fluorescent Anti-Rabbit IgG - DyLight^® 594 conjugate used at a dilution of 1:100.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho SMC1 (S966) Antibody, Affinity Purified: <B>Cat. No. PLA0015</B>: Detection of Phospho SMC1 (S966) by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells that were treated with NCS (+; 200 ng/mL; 1 hour) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-Phospho SMC1 (S966) Antibody <B>PLA0015</B> used at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, Rabbit Anti-SMC1 Antibody <B>PLA0017</B> was used.

Immunoblotting
Rabbit Anti-Phospho SMC1 (S966) Antibody, Affinity Purified: Cat. No. PLA0015: Detection of Phospho SMC1 (S966) by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells that were treated with NCS (+; 200 ng/mL; 1 hour) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-Phospho SMC1 (S966) Antibody PLA0015 used at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, Rabbit Anti-SMC1 Antibody PLA0017 was used.