ImmunofluorescencePOLR2A and phospho-BRCA1 [S1280] PLA Antibody Kit (
Cat. No. PLAK0042): Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. Goat Anti-Human POLR2A (component
Cat. No. PLA0292 GeneID 5430) and Rabbit Anti-Human phospho-BRCA1[S1280] (component
Cat. No. PLA0003 GeneID 672). Image merged from DAPI (2 ms), FITC/Green (25 ms) and TRITC/Orange (250 ms) exposures, 40× magnification.
ImmunofluorescenceNegative control proximity ligation assay performed using component
PLA0003 paired with purified goat IgG isolated from normal, clinically healthy animals that have not been exposed to specific antigen.
ImmunofluorescenceNegative control proximity ligation assay performed using component
PLA0292 paired with purified rabbit IgG isolated from normal, clinically healthy animals that have not been exposed to specific antigen.