ImmunofluorescenceCHK1 and phospho-BRCA1 [S1524] PLA Antibody Kit (
Cat. No. PLAK0020): Secondary-conjugate Duolink In Situ PLA in HeLa cells. Goat Anti-Human CHK1 (component
Cat. No. PLA0055 GeneID 1111) and Rabbit Anti-Human phospho-BRCA1[S1524] (component
Cat. No. PLA0002 GeneID 672). Image merged from DAPI (2 ms), FITC/Green (25 ms) and TRITC/Orange (250 ms) exposures, 40× magnification. Control images may not have counterstained cytoPLAsm.
ImmunofluorescenceNegative control proximity ligation assay performed using component
PLA0002 paired with purified goat IgG isolated from normal, clinically healthy animals that have not been exposed to specific antigen.
ImmunofluorescenceNegative control proximity ligation assay performed using component
PLA0055 paired with purified rabbit IgG isolated from normal, clinically healthy animals that have not been exposed to specific antigen.