Using the GSK3beta assay kits or Western blot, GSK3beta phosphorylation at Ser9 is detected in insulin-treated MCF7 cells (+), compared with cells treated with UCN-01 (-).
Using the AKT 1/2/3 assay kits, a significant increase in AKT 1/2/3 phosphorylation at Ser473 is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with untreated cells (-).
Using the p70S6K assay kits, a significant reduction of p70S6K phosphorylation at Thr389 is detected in MCF7 cells treated with wortmannin (-) for 3 hours, compared with untreated cells (+).
Using the S6RP assay kits or Western blot, S6RP phosphorylation at Ser240/244 is detected in insulin-treated MCF7 cells (+), compared with MCF7 cells treated with a combination of rapamycin, UCN-01 and U0126 (-).