Example of a typical GAPDH cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
Example of a typical GAPDH recombinant protein standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of GAPDH are normalized and plotted.
Cell line analysis for GAPDH from 5 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).