ERK 1/2 (pT202/Y204) + AKT 1/2/3 (pS473) + CREB1 (pS133) PhosphoTracer ELISA Kit
| Name | ERK 1/2 (pT202/Y204) + AKT 1/2/3 (pS473) + CREB1 (pS133) PhosphoTracer ELISA Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab119679 |
| Category | ELISA Kit |
| Assay Type | Semi-quantitative |
| Sample Type | Cell culture extracts |
| Detection | Fluorescent |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Mouse, Hamster, Human, Rat |
| Gene | MAPK3 |
| Supplier Page | Shop |
Product images
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours (-), compared with cells treated with EGF for 10 min (+).
Using the AKT 1/2/3 assay kits, a significant increase in AKT 1/2/3 phosphorylation at Ser473 is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with untreated cells (-).
Using the CREB1 assay kits or Western blot, CREB1 phosphorylation at Ser133 is detected in EGF-treated A431 cells (+), compared with untreated cells (-).