The HeLa lysate range was prepared as described in Section 9 of the protocol using a dilution factor of 1.4. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Background subtracted data from duplicate measurements are plotted.
Background subtracted data from duplicate measurements are plotted.
The levels of CIV in 143B-derived Rho0 cells were measured in comparison to the levels of 143B-Wild type cells by loading in triplicate 300µg/mL of protein per well. The concentrations of CIV were interpolated from a Complex IV standard curve of HeLa cell extract and graphed in percent relative to Complex IV expression in HeLa cell extract. The concentration of Complex IV in the Rho0 cell line was not detectable.
The Levels of CIV deficient fibroblasts with a compound heterozygote mutation in the SURF1 gene >c.311insAT_delTCTGCCAGCC, c.751C>Tp.R251X (kind gift from Dr. Garry K. Brown, University of Oxford) were measured in comparison to the levels of a control primary fibroblast by loading in triplicate 300µg/mL of total protein per well. The concentrations of CIV were interpolated from a standard curve of HeLa cell extract and graphed in percent relative to Complex IV expression in the HeLa cell extract. The concentration of Complex IV in the cell line with the SURF1 mutation was 16% from the control fibroblast.