All lanes : Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DNAJC11 with ab183518 at 1/250 dilution. The slide is counterstained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling DNAJC11 with ab183518 at 1/90 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.