All lanes : Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : Hela cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of DNAJA4 in 2% paraformaldehyde HeLa cells using ab185553 at 1/300 dilution (pink). Secondary antibody: Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green).
Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2: PBS used instead of Human fetal brain lysates. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer: 5% NFDM/TBST.