Atg13 (E1Y9V) Rabbit mAb

Name	Atg13 (E1Y9V) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	13468
Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	E1Y9V
Applications	WB IP ICC/IF
Species Reactivities	Human
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn230 of human Atg13 protein.
Description	Rabbit Monoclonal
Gene	ATG13
Supplier Page	Shop

Product images

Western blot analysis of extracts from various cell lines using Atg13 (E1Y9V) Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Atg13 (hAtg13-Myc/DDK; +), using Atg13 (E1Y9V) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Atg13 siRNA I #12043 (+), using Atg13 (E1Y9V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Atg13 (E1Y9V) Rabbit mAb confirms silencing of Atg13 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Immunoprecipitation of Atg13 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype control #3900 (lane 2) or Atg13 (E1Y9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Atg13 (E1Y9V) Rabbit mAb.

Confocal immunofluorescent analysis of PANC-1 cells, untreated (left) or nutrient-starved with Earle's balanced salt solution (3 hr; right), using Atg13 (E1Y9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).