All lanes : Anti-DCK antibody [16E12] (ab118994) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DCK cDNALysates/proteins at 5 µg per lane.
HEK293T cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118994 at 1/100, and then analyzed by flow cytometry.
Flow cytometric analysis of HeLa cells, using ab118994 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometric analysis of Jurkat cells, using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).