All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/50000 dilutionLane 1 : Human fetal liver cell lysateLane 2 : MCF7 cell lysateLane 3 : 293T cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of HeLa cells labeling DCI with ab186752 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling DCI with ab186752 at 1/240 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7cells labeling DCI with ab186752 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/10000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution