Anti-Daxx antibody [E94]

Name	Anti-Daxx antibody [E94]
Supplier	Abcam
Catalog	ab32140
Prices	$391.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	E94
Applications	WB IHC-P ICC/IF FC
Species Reactivities	Human
Antigen	A synthetic peptide corresponding to residues of human DAXX
Description	Rabbit Monoclonal
Gene	DAXX
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab32140 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32140 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Overlay histogram showing HeLa cells stained with ab32140 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32140, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Daxx antibody [E94] (ab32140) at 1/5000 dilution + Hela cell lysate

Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.

Product References

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Breaking human cytomegalovirus major immediate-early gene silence by vasoactive - Breaking human cytomegalovirus major immediate-early gene silence by vasoactive

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New insights into the role of the subnuclear structure ND10 for viral infection. - New insights into the role of the subnuclear structure ND10 for viral infection.

Tavalai N, Stamminger T. Biochim Biophys Acta. 2008 Nov;1783(11):2207-21. doi: