Anti-Cyclin B1 antibody [V152]

Name	Anti-Cyclin B1 antibody [V152]
Supplier	Abcam
Catalog	ab72
Prices	$401.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	V152
Applications	SDS-PAGE ICC/IF ICC/IF IHC-P FC WB IHC-F IHC-P IHC-F
Species Reactivities	Human, Mouse
Antigen	His-tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HCl and purified on Nickel beads
Description	Mouse Monoclonal
Gene	CCNB1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Cyclin B1 antibody [V152] (ab72) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951)Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ab72 staining in Human Normal Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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Product References

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