Anti-Ctip1 [EPR14943-44] antibody (ab191401) at 1/50000 dilution + 293 cell lysate at 10 µgSecondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue labeling Ctip1 with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling Ctip1 with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Immunofluorescence analysis of, paraformaldehyde-fixed, rat C6 cells labeling Ctip1 with ab191401 at a 1/450 dilution (4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-BCL11A at a 1/450 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.