ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal.See Abreview

ICC/IF image of ab5097 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS Tween 0.01% + donkey serum 1%) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/500) was used as the secondary antibody.See Abreview