ab102989 staining CREB3L2 in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1.5% serum for 1 hour at 25°C and then incubated with ab102989 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an undiluted biotin conjugated goat polyclonal.See Abreview

Immunofluorescence analysis of U2-OS, staining CREB3L2 with ab102989. Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 in PBS and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA in PBS) for 12 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.See Abreview