Anti-Cortisol Binding Globulin antibody
| Name | Anti-Cortisol Binding Globulin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab96270 |
| Prices | $370.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant protein fragment corresponding to a region within amino acids 105 and 353 of Human Cortisol Binding Globulin (NP_001747) |
| Description | Rabbit Polyclonal |
| Gene | SERPINA6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Cortisol Binding Globulin antibody (ab96270) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using ab96270 at 1/200 dilution. Lower image merged with DNA probe.
IHC image of Cortisol Binding Globulin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96270, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.