Formalin fixed paraffin embedded human normal Skin and basal cell carcinoma/solar elastos stained with ab6308 at 1/800 for 30min.

ab6308 staining pig small intestine tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) (ab64214) prior to incubating with ab6308, diluted 1/800, for 1 hour at 20°C.  A HRP-conjugated goat anti-mouse antibody was used as the secondary.The use of an antibody diluent replaced a blocking step.See Abreview

ab6308 staining human skin tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to incubating with ab6308, diluted 1/800, for 1 hour at 20°C.  An Alexa Fluor® 488 conjugated goat anti-mouse (ab150113) antibody was used as the secondary.The use of an antibody diluent replaced a blocking step.See Abreview

ab6308 at a 1/1000 dilution staining in rabbit polymer scaffold with stem cells by Immunocytochemistry/ Immunofluorecence, incubated for 2 hours at 20°C. PFA fixed. Permeabilized using 0.5% Triton X-100. Blocked with 3% BSA for 2 hours at 4°C. Secondary used at 1/1000 dilution monoclonal donkey anti-mouse IgG (H+L) conjugated to FITC.1st column: DAPI stain (blue)2nd column: collagen type I (green)3rd column: merge imageSee Abreview

Anti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µgSecondaryAn HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.