IHC image of Collagen I staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88147, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Collagen I antibody [3G3] (ab88147) at 1 µg/ml + immunogen (88 aa recombinant fragment with a proprietary tag of 26kDa) at 0.2 µgSecondaryGoat anti-Mouse IgG at 1/5000 dilution

Anti-Collagen I antibody [3G3] (ab88147) at 1 µg/ml + NIH 3T3 cell lysate at 25 µgSecondaryGoat anti-Mouse IgG at 1/5000 dilution

Detection limit for ab88147 is 0.03 ng/ml as a capture antibody.