Anti-CLCA3 antibody
| Name | Anti-CLCA3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab46512 |
| Prices | $380.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF IP Electron microscopy |
| Species Reactivities | Mouse |
| Antigen | Synthetic peptide: EKNHNQEAPNDQNQR , corresponding to amino acids 253-267 of Mouse CLCA3 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | Clca1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin-fixed, paraffin-embedded mouse colon tissue sections stained with anti-CLCA3 ab46512 used at 1/8000 dilution.
Anti-CLCA3 antibody (ab46512) at 1/500 dilution + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab46512 stained MEF1 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46512, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Differential effects of rapamycin and dexamethasone in mouse models of - Differential effects of rapamycin and dexamethasone in mouse models of
Mushaben EM, Brandt EB, Hershey GK, Le Cras TD. PLoS One. 2013;8(1):e54426.
Antibody to mCLCA3 suppresses symptoms in a mouse model of asthma. - Antibody to mCLCA3 suppresses symptoms in a mouse model of asthma.
Song L, Liu D, Wu C, Wu S, Yang J, Ren F, Li Y. PLoS One. 2013 Dec 9;8(12):e82367.
Epithelial EGF receptor signaling mediates airway hyperreactivity and remodeling - Epithelial EGF receptor signaling mediates airway hyperreactivity and remodeling
Le Cras TD, Acciani TH, Mushaben EM, Kramer EL, Pastura PA, Hardie WD, Korfhagen TR, Sivaprasad U, Ericksen M, Gibson AM, Holtzman MJ, Whitsett JA, Hershey GK. Am J Physiol Lung Cell Mol Physiol. 2011 Mar;300(3):L414-21. doi:
Rapamycin attenuates airway hyperreactivity, goblet cells, and IgE in - Rapamycin attenuates airway hyperreactivity, goblet cells, and IgE in
Mushaben EM, Kramer EL, Brandt EB, Khurana Hershey GK, Le Cras TD. J Immunol. 2011 Dec 1;187(11):5756-63.
Surfactant protein C-deficient mice are susceptible to respiratory syncytial - Surfactant protein C-deficient mice are susceptible to respiratory syncytial
Glasser SW, Witt TL, Senft AP, Baatz JE, Folger D, Maxfield MD, Akinbi HT, Newton DA, Prows DR, Korfhagen TR. Am J Physiol Lung Cell Mol Physiol. 2009 Jul;297(1):L64-72. doi: