All lanes : Anti-CHMP6 antibody (ab76929) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.