
All lanes : Anti-CFHR5 antibody (ab175254) at 1/100 dilutionLane 1 : CEM cell lysateLane 2 : K562 cell lysateLysates/proteins at 15 µg per lane.

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human hepatocarcinoma tissue, labeling CFHR5 using ab175254 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometry analysis of CEM cells labeling CFHR5 (green, right histogram), using ab175254 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.