Anti-CEP55 antibody [EPR11944(B)]

Name	Anti-CEP55 antibody [EPR11944(B)]
Supplier	Abcam
Catalog	ab170414
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR11944(B)
Applications	IHC-P WB ICC/IF ICC/IF FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CEP55
Description	Rabbit Monoclonal
Gene	CEP55
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/5000 dilution + SW480 Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

All lanes : Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution (unpurified)Lane 1 : SW480 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysatedeveloped using the ECL techniquePerformed under reducing conditions.