Flow cytometric analysis of BOSC23 cells transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). ab167477 was used at 1.2 µg/106 cells and detection was with a PE- conjugated secondary antibody.
Immunohistochemical analysis of paraffin-embedded adult transgenic mouse cochlea tissue with a Human CEACAM16 gene, labeling CEACAM16 with ab167477 at 4 µg/ml. Binding of biotinylated antibody was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole. OH: Outer hair cells, TM: Tectorial membrane, RM: Reissner’s membrane.
BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, ab167477 was tested on all CEACAM transfectants at 1.2 µg/106 cells. A positive signal was obtained only with CEACAM16 transfected cells (red curve).
SDS-PAGE analysis of ab167477 at 2 µg.