ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence.SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.