Anti-CDC42 antibody
| Name | Anti-CDC42 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64533 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human, Yeast, Pig, Chimpanzee, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human CDC42 |
| Description | Rabbit Polyclonal |
| Gene | CDC42 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 5 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab64533 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% pfa fixed (10 min) HeLa cells at 5µg/ml.
All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/mlLane 1 : Human CDC42 full length protein (ab87713) at 0.1 µgLane 2 : Human CDC42 full length protein (ab87713) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Product References
Competing targets of microRNA-608 affect anxiety and hypertension. - Competing targets of microRNA-608 affect anxiety and hypertension.
Hanin G, Shenhar-Tsarfaty S, Yayon N, Yau YH, Bennett ER, Sklan EH, Rao DC, Rankinen T, Bouchard C, Geifman-Shochat S, Shifman S, Greenberg DS, Soreq H. Hum Mol Genet. 2014 Sep 1;23(17):4569-80.
An integrative omics strategy to assess the germ cell secretome and to decipher - An integrative omics strategy to assess the germ cell secretome and to decipher
Chalmel F, Com E, Lavigne R, Hernio N, Teixeira-Gomes AP, Dacheux JL, Pineau C. PLoS One. 2014 Aug 11;9(8):e104418.
Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2 - Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2
Hoeke L, Sharbati J, Pawar K, Keller A, Einspanier R, Sharbati S. PLoS One. 2013 Jun 24;8(6):e67300.