Overlay histogram showing Jurkat cells stained with ab92726 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92726, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ab92726, at a 1/100 dilution, staining CD9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemisty.

ab92726, at a 1/100 dilution, staining CD9 in paraffin embedded Human endometrial carcinoma tissue by Immunohistochemisty.

ab92726, at a 1/100 dilution, staining CD9 in paraffin embedded Human tonsil tissue by Immunohistochemisty.

ab92726 showing positive staining in Papillary carcinoma of thyroid gland tissue.

ab92726 showing positive staining in Normal kidney tissue.

ab92726 showing positive staining in Astrocytoma tissue.

ab92726 showing positive staining in Normal brain tissue.

ab92726 showing positive staining in Papillary carcinoma of thyroid gland tissue.

ab92726 showing positive staining in Normal kidney tissue.

ab92726 showing positive staining in Astrocytoma tissue.

ab92726 showing positive staining in Normal brain tissue.