Anti-CD46 antibody - C-terminal (ab175096) at 1/100 dilution + CEM lysate at 35 µg
Immunohistochemistry analysis of formalin fixed and paraffin embedded Human hepatocarcinoma tissue labeling CD46 using ab175096 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram) labeling CD46 using ab175096 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CD46 Antibody (C-term) (ab175096) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).