ICC/IF image of ab11024 staining of human platelet cells. The sections were incubated in 10% serum to block non-specific protein-protein interactions and in 0.2% triton X to permeabilise the cells. The sections were then incubated with ab11024 (1:400) for one hour at 20°C, followed by alexa 488 conjugated secondary antibody (green). Platelets were counterstained with rhodamine phalloidin to outline the cellular cytoskeleton (red).See Abreview

Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.