Flow analysis of CD4 in mouse splenocytes, using 0.05 ug of ab86859 for 106 cells. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti CD4 antibody ab86859.
Overlay histogram showing Mouse thymocyte cells stained with ab86859 (blue line). The cells dissociated from Thymus tissue into a single cell suspension. Cells were gated on lymphocytes. They were incubated the primary antibody (ab86859, 1µg/1x106 cells) for 30 min. Isotype control antibody (red histogram) was a FITC-conjugated CD8 antibody used under the same conditions. See Abreview