Anti-CD20 antibody (ab88247) at 1 µg/ml + Human spleen at 25 µgSecondaryGoat Anti-Mouse IgG at 1/2500 dilutionObserved band size : 33 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-CD20 antibody (ab88247) at 1 µg/mlLane 1 : CD20 transfected lysateLane 2 : Non-transfected lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG at 1/2500 dilutionObserved band size : 33 kDa (why is the actual band size different from the predicted?)
ab88247, at 3µg/ml, staining human CD20 in lymph node, using Immunohistochemistry, Formalin/PFA-fixed paraffin embedded tissue.
FACS analysis of negative control 293 cells (Black) and CD20 expressing 293 cells (Green) using ab88247.