All lanes : Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] (ab124883) at 1/1000 dilutionLane 1 : HUVEC cell lysate untreatedLane 2 : HUVEC cell lysate treated with PervanadateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Flow cytometric analysis of permeabilized HUVEC cells, untreated (green) or pervanadate treated (red) using ab124883 at 1/10 dilution, and pervanadate-treated HUVEC cells using the same antibody preincubated with phospho-Caveolin 2 Phospho (pY19) peptide (blue) or non-phospho- Caveolin-2 Phospho (pY19) peptide (orange).
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD